THE 2-MINUTE RULE FOR NAD+ SUPPLEMENTS FOR ENERGY AND METABOLISM

Subsequent, we addressed cells with diverse concentrations of NAD+. When included for twenty-four h at 80 µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Details Fig. 1k,l). In addition, sixteen µM of NAD+ was ample to cut back fork pace by ~40% in HeLa cells without affecting the overall